Construction of Sjcb2 DNA Vaccine and its Expression in HeLa Cells
- VernacularTitle:Sjcb2 DNA疫苗的构建及其在HeLa细胞中的表达
- Author:
Yongxuan HU
;
Jianhua XIAO
;
Jiafang HUANG
;
Qiulin YANG
- Publication Type:Journal Article
- Keywords:
Schistosoma japonicum;
Cathepsin B Endopeptidase;
DNA vaccine;
Eukaryotic expression
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1997;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
A recombinant plasmid containing cathepsin B endopeptidase of Schistosoma japonicum(Sjcb2)was constructed,indentified by PCR,restrictive enzyme,digestion and DNA sequencing,and expressed into mammalian cells.Immunochemistry examination showed that the Sjcb2 gene can be expressed in the eukaryotic system,providing a basis for the development of schistosome DNA vaccine.
