Amelioration in cardiac function with Ad_5-HGF transference in postinfarction heart failure swine
- VernacularTitle:肝细胞生长因子治疗心肌梗死后心力衰竭的实验研究
- Author:
Wei WANG
;
Zhijian YANG
;
Dongchao MA
- Publication Type:Journal Article
- Keywords:
Gene therapy;
Heart failure, congestive;
Myocardial infarction;
Collateral circulation
- From:
Chinese Journal of Interventional Cardiology
2003;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the ameliorative effect on postinfarction heart failure of Ad_5-HGF (adenovirus-mediated human hepatocyte growth factor) in swine models. Methods Totally 12 young swine were randomly divided into 2 groups which were: Ad_5-HGF group (treatment group) and null- Ad_5 group (control group). At four weeks and seven weeks after left anterior descending coronary artery ligation in swine hearts, coronary angiography and Gate cardiac perfusion imaging were performed to evaluate the degree of collateral artery growth (Rentorp), cardiac perfusion, and heart function. The expression of HGF was examined by ELISA. Immunohistochemistry was performed to illustrate the angiogenesis of SMA~+ vessels. Results (1) In comparison with the null- Ad_5 group, high expression of human HGF was observed in the myocardium of the Ad_5-HGF group. (2) The improvement in LVEF was more obvious in the Ad_5-HGF group than the control group in 7 weeks. Cardiac perfusion was significantly improved in the Ad_5-HGF group. (3) In the Ad_5-HGF group, growth of collateral blood vessels and also the number of SMA~+ vessels per mm~2 were significantly greater. Conclusion (1) High expression of human HGF is observed in the myocardium through intracoronary transfection. (2) HGF can improve collateral artery formation and ameliorate heart function. (3) HGF can increase the number of vessels that are coated with SMC.
