The research of serum-DNA IgH and T-cell receptor gama gene rearrangement in lymphogenous malignant patients
- VernacularTitle:淋巴系统恶性肿瘤患者血清游离DNA含量与重排基因检测及其临床意义
- Author:
Kaili YAN
;
Weihua REN
;
Xiucai XU
;
Zhimin ZHAI
- Publication Type:Journal Article
- Keywords:
Serum-DNA;
Gene rearrangement;
Acute lymphocyte leukemia;
Lymphoma
- From:
Chinese Journal of Laboratory Medicine
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the diagnostic significance of detecting serum-DNA concentration and clonal gene rearrangement in lymphogenous malignant patients.Methods Serum-DNA in 72 diagnosed patients were measured and analyzed by SPSS11.0.Serum and mono-nuclear cells samples were collected. IgH CDRⅢ, TCR?V9 region were amplified by PCR.Results The serum-DNA concentration of disease groups were (418?172)?g/ml,(426?192)?g/ml, (388?170)?g/ml and(400?110)?g/ml, each was higher than those of control group(77?47)?g/ml(P0.05).Conclusion The concentration of serum-DNAwere higher in lymphogenous malignant patients and tumor associated DNA could be easily detected, so they have important diagnostic value in lymphogenous malignant patients.
