Applying Lactacystin,a selective proteasome inhibitor,to set up the rat model of Parkinson's disease with Lewy body
- VernacularTitle:应用蛋白酶体抑制剂Lactacystin建立有Lewy体的帕金森病大鼠模型
- Author:
Kezhong ZHANG
;
Yuping JIANG
;
Xinsheng DING
- Publication Type:Journal Article
- Keywords:
Lactacystin;
proteasome;
?-synuclein;
Lewy body
- From:
Journal of Clinical Neurology
2001;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To set up the Parkinson's disease(PD)rat model with pathologic characteristic of Lewy body in nigral cells.Methods SD rats were injected respectively with 8 mg Lactacystin(Lactacystin group),sodium saline(NS group)and 12 mg 6-OHDA(6-OHDA group)by stereotaxic unilateral injection into the pars compacta of substantia nigral.The spontaneous and apomorphine-induced contralateral behaviors of rats were observed.The changes of midbrain histology were viewed by microscope;expression of ?-synuclein and tyrosine hydroxylase(TH)positive cells were investigated by immunohistochemistry.The contents of dopamine and homovanillic acid in striatum were determined.Results Rats of NS group did not display abnormal behavior.The animals treated with Lactacystin developed progressively bradykinesia,hypokinesia,tremor,contralateral head tilting,and displayed apomorphine-induced contralateral rotation behavior;3 weeks later the number of TH positive cells were decreased by 83.29% compared with NS group(P
