Tumor necrosis factor-? regulates the effect of glutamine on protein synthesis in rats' skeletal muscle
- VernacularTitle:肿瘤坏死因子对谷氨酰胺调控大鼠骨骼肌蛋白质合成的影响
- Author:
Jihong ZHOU
- Publication Type:Journal Article
- Keywords:
Glutamine;
Fractional synthesis rate;
Stable isotopes;
[L-15N] Leucine;
Tumor necrosis factor-?
- From:
Journal of Medical Postgraduates
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To find the regulations of glutamine(Gln) metabolism by tumor necrosis factor-?(TNF-?).Methods:Thirty male SD rats were assigned into three groups: A(total parenteral nutrition,TPN),B(TPN+Gln),C(TPN+ TNF-?+Gln).Besides all rats were supported by isonitrogenous and isocaloric TPN solutions for 3 days,rats in group B were supplied with Gln(in the form of alanylglutamine) for 3 days at the rate of 0.3 g/(kg?d),rats in group C were infused intravenously with TNF-? at the rate of 5 ?g/(kg?h) within the last 24 hours and also supplemented with Gln for three days at the same rate as in group B.At the last 0.5 hour,all animals were mainlined with \leucine at the dosage of 1.0 mmol/kg.TNF-? levels in plasma,concentrations of Gln in plasma and skeletal muscle were measured as the experiment ended after 72 hours,and the fractional synthesis rate(FSR) were also assessed.Results:The Gln concentrations in plasma and skeletal muscle increased markedly from group A,B to C.The levels of FSR decreased remarkably along with the sequence from group B,C to A.The statistical differences were significant(P
