Evaluation of specificity and sensitivity of three kinds of real-time PCR instruments
- VernacularTitle:三种PCR仪的特异性及灵敏度评价
- Author:
Zheng ZENG
;
Dan LIU
;
Weibo GONG
;
Chongwen SI
- Publication Type:Journal Article
- Keywords:
real-time PCR;
American Applied Biosystems;
clinical trial
- From:
Chinese Medical Equipment Journal
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To evaluate the specificity and sensitivity of ABI 7000,7300 and 7500 real-time PCR instruments.Methods:Random,double-blind clinical trial;We selected 30 patients with HBsAg and HBV DNA positive for positive group and 30 health volunteers with HBsAg and HBV DNA negative for negative group.We also selected three of HBV DNA positive and negative samples for repeat test.Results:The 30 samples of positive group were detected HBV DNA.The 30 samples of negative group were not detected HBV DNA.However,in positive group,the HBV DNA levels were different.The related index of ABI 7000 with ABI 7300,ABI 7500 and ABI 7300 with ABI 7500 was 0.89,0.89 and 0.98,respectively.The HBV DNA level was highest detected with ABI 7300 instrument,and then with ABI 7000 instrument.The lowest level was detected with ABI 7500 instrument.There was significance among these instruments(p
