Generation of Adiponectin Gene Knock-out and LacZ Gene Knock-in Mouse Model
- VernacularTitle:Adiponectin基因剔除LacZ基因敲入小鼠模型的建立
- Author:
Weihua REN
;
Xihua LI
;
Fang WANG
;
Jianou QIAO
;
Suying DANG
;
Hui KONG
;
Long WANG
;
Shunyuan LU
;
Xia SUN
;
Guojiang XU
;
Jiliang FU
;
Jian FEI
;
Zhugang WANG
- Publication Type:Journal Article
- Keywords:
adiponectin, gene knockout, LacZ, gene knock-in
- From:
Progress in Biochemistry and Biophysics
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Adiponectin is an adipocyte-derived secretory protein. It was found to be associated with insulin resistance, inflammation and arteriosclerosis. To further study the biological function and expression of adiponection in vivo, adipoenctin gene knock-out and LacZ gene knock-in mouse model was constructed. Gene targeting strategy was designed to replace part of exon 2 and exon 3 of adiponectin gene with full length LacZ gene in frame with remaining upstream ATG and signal peptide sequence of exon 2. The targeting vector (Adipo-LacZ-XpPNT) was constructed and verified by restriction enzyme digestion and sequencing. CJ7 ES cells were transfected with targeting vector linearized by NotⅠ digestion, selected in the medium containing both G418 and ganciclovoir. Resistant clones were screened by PCR and further confirmed by Southern blot for correct homologous recombinants. Chimera mice were obtained by routing microinjection of homologous recombined ES cells into blastocysts. After mating, mice heterozygous and further homozygous for adiponectin knockout and LacZ gene knock-in were established. Expression of both endogenous adiponectin and exogenous LacZ gene in mouse tissues and sera were detected by RT-PCR, Northern-blot, Western blot and ELISA. The results show that adiponectin was disrupted at both mRNA and protein levels. LacZ gene is expressed exclusively in adipose tissue of mutant mice. Its expression profile is identical to endogenous adiponection. Unexpectedly, LacZ activity could not be detected in both adipose tissue and serum although LacZ protein can be detected in adipose tissue but not in serum of mutant mice. In conclusion, mice homozygous for adiponectin knockout and LacZ gene knock-in have been successfully constructed. Mutant mice display LacZ expression profile identical to endogenous adiponectin albeit neither LacZ activity nor protein can be detected in serum of mutant mice.
