Role of P38 MAPK activation in glucose-induced microvascular hyperpermeability of rats
- VernacularTitle:P38 MAPK激活在高血糖致大鼠微血管通透性增高过程中的作用
- Author:
Yanjun ZHU
;
Qiang LI
;
Bo CHEN
;
Xuliang HUANG
;
Qiaobing HUANG
- Publication Type:Journal Article
- Keywords:
diabetes mellitus;
hyperglycemia;
microvascular permeability;
cystoskeleton;
P38 MAPK
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of P38 mitogen-activated protein kinase(P38 MAPK) activation in high level glucose-induced microvascular hyperpermeability.Methods Rats were induced to diabetis by intraperitoneal injection of streptozotocin(STZ).The rats were divided into 5 groups,including normal,diabetes,control,MKK6b(A) and MKK6b(E) groups.The permeability coefficient to albumin(Pa) was measured in venules of in vivo mesenterium using a fluorescence ratio technique;Morphological changes of microvascular endothelial cell were monitored by observing fluorescence of F-actin stained with rhodamine-phalloidin.Results The permeability of diabetic rats was obviously increased.The activation of P38 MAPK by MKK6b(E) could increase microvascular permeability in normal rats,and the inhibition of P38 MAPK by MKK6b(A) could inhibit hyperpermeability of diabetic rats.Conclusion The activation of P38 MAPK induced by hyperglycemia may play a role in diabetic microvascular hyperpermeability.
