Effect of cell adhesion on vasodilator-stimulated phosphoprotein phosphorylation
- VernacularTitle:细胞黏附对血管扩张刺激磷蛋白磷酸化的影响
- Author:
Tingting WANG
- Publication Type:Journal Article
- Keywords:
cell adhesion;
VASP;
PKA
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of Vasodilator-stimulated Phosphoprotein phosphorylation which was dynamically regulated by cell adhesion to extracellular matrix,and to investigate the roles of VASP in the process of tumor cell adhesion and de-adhesion.Methods HeLa cells were divided into four groups: stably adherent cells,cells held in suspension,cells replated onto dishes coated with collagen I and positive control(cells treated with Forskolin).Changes in cell morphology were observed in inverted phase contrast microscope.Phosphorylation of VASP was detected by Western blot,and PKA activity was measured by PKA assay kit.(Results In) untreated,stably adherent cells,VASP was predominantly in the unphosphorylated form; Detachment of cells stimulated PKA activity and induced PKA-dependent phosphorylation of VASP;VASP was phosphorylated gradually following reattachment but returned to base level of phosphorylation during active cell spreading.Conclusion During the process of HeLa cell adhesion and de-adhesion,dynamical changes of VASP from the form of phosphorylation to dephosphorylation via PKA have been observed.
