Effect of sex hormones on insulin sensitivity shown by 3T3-L1 preadipocytes and adipocytes
- VernacularTitle:性激素对3T3-L1前脂肪细胞和脂肪细胞胰岛素敏感性的影响
- Author:
Jing WU
;
Hongwei WANG
;
Yu WEN
;
Xiufen HU
- Publication Type:Journal Article
- Keywords:
3T3-L1;
estradiol;
testosterone;
glucose uptake;
insulin resistance
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different sex hormones on insulin-stimulated glucose uptake on cultrured 3T3-L1 preadipocytes and adipocytes.Methods We treated cultured 3T3-L1 preadipocytes and adipocytes with different concentrations of 17?-Estradiol or testosterone and the effects were assessed as 2-deoxy glucose uptake.Results After incubation with 17?-Estradiol or testosterone,the ability of insulin-stimulated glucose transport was statistically significantly reduced.Dosage response study demonstrated that(10~(-7) mol/L) testosterone or(10~(-8) mol/L) 17?-Estradiol can induce insulin resistance(P
