Glucose regulates protein 75 expression in PC12 cell line under glucose deprivation
- VernacularTitle:葡萄糖调节蛋白75在缺糖损伤PC12细胞中的表达
- Author:
Xiaodong SONG
;
Shaofeng JIANG
;
Peng MA
;
Yuyong WU
;
Yan LIU
;
Ji ZUO
- Publication Type:Journal Article
- Keywords:
GRP75;
PC12 cell;
glucose deprivation;
apoptosis
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the effect of glucose regulating protein 75 expression in PC12 cells under glucose deprivation.Methods MTT method was used to monitor the cell viability.The leakage of LDH was represented as the index of cell injury.Flow cytometry was applied for cell cycle analysis,apoptosis and necrosis.Using RT-PCR detected and quantified mRNA.Western blot to measure relative amounts of the protein.Results Cell viability decreased and the LDH leakage increased.While the treated PC12 cells had two main forms of apoptosis and necrosis.GRP75 mRNA and protein levels were upregulated.Conclusion GRP75 has protective function during glucose deprivation.
