Isolating method of rat pancreatic islets and affect factors for its activity
- VernacularTitle:大鼠胰岛分离技术的建立及胰岛活性的影响因素
- Author:
Baocheng CHANG
;
Hong ZHANG
;
Ning ZHENG
;
Wei ZHAO
;
Qiumei ZHANG
;
Lijun WEI
;
Peihua FANG
- Publication Type:Journal Article
- Keywords:
islets of Langerhans;
activity;
BSA;
glucose concentration of medium;
cultured hours
- From:
Basic & Clinical Medicine
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the factors which affect the activity of isolated islets,and to construct a steady and effective isolation method of rat pancreatic islets.Methods Pancreatic islets were isolated by collagenase method.The glucose-stimulation of insulin secretion(GSIS) in different conditions was performed by batch incubation method and measured by RIA.Results GSIS was improved by BSA;There was a evident decrease of insulin secretion in cultured islets of 7 days,however,no difference was observed between the freshly isolated islets group and the 1～5days cultured islets groups.Compared to the groups of(5.5 mmol/L) and(25 mmol/L) glucose in the culture medium,(11.1 mmol/L) glucose group stimulated higher insulin release.Conclusion BSA,glucose concentration of RPMI1640 medium and the culture period are related to the activity of isolated islets.
