Comparative Analysis of Human Epidermal and Peripheral Blood gammadelta T Cell Cytokine Profiles.
- Author:
Kwangmi KIM
1
;
Jiyeon HAN
;
Tae Ryong LEE
;
Dong Wook SHIN
;
Hak CHANG
;
A Ri CHO
;
Soon Jin CHOI
;
Seong Jin JO
;
Ohsang KWON
Author Information
- Publication Type:Original Article
- Keywords: Epidermis; Interleukin-4; Interleukin-13; gammadelta T cells
- MeSH: Epidermis; Granulocyte-Macrophage Colony-Stimulating Factor; Healthy Volunteers; Homeostasis; Humans; Inflammation; Interferon-gamma; Interleukin-13; Interleukin-2; Interleukin-4; Macrophages; Skin; Skin Diseases; T-Lymphocytes
- From:Annals of Dermatology 2014;26(3):308-313
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Human epidermal gammadelta T cells are known to play crucial roles in the defense and homeostasis of the skin. However, their precise mechanism of action in skin inflammation remains less clear. OBJECTIVE: In this study, we analyzed the cytokine expression profile of human epidermal gammadelta T cells and compared it to that of peripheral blood gammadelta T cells to investigate the specific activity of epidermal gammadelta T cells in modulating skin inflammation. METHODS: We isolated gammadelta T cells from epidermal tissue or peripheral blood obtained from healthy volunteers. Isolated gammadelta T cells were stimulated using immobilized anti-CD3 antibody and interleukin-2 plus phytohaemagglutinin, and were then analyzed using a cytokine array kit. RESULTS: Both epidermal and peripheral blood gammadelta T cells produced comparable levels of granulocyte-macrophage colony-stimulating factor, I-309, interferon-gamma, macrophage migration inhibitory factor, macrophage inflammatory protein-1alpha, and chemokine (C-C) ligand 5. The epidermal gammadelta T cells produced significantly higher levels of interleukin-4, -8, -13, and macrophage inflammatory protein-1beta than the peripheral blood gammadelta T cells did. Notably, the epidermal gammadelta T cells produced several hundred-fold higher levels of interleukin-13 than interleukin-4. CONCLUSION: These results suggest that the epidermal gammadelta T cells have a stronger potential to participate in the Th2-type response than the peripheral blood gammadelta T cells do. Furthermore, epidermal gammadelta T cells might play an important role in the pathogenesis of Th2-dominant skin diseases because of their active production of interleukin-13.
