Inhibitory effect of particle ~(125)I on proliferation of intracranial glioma cells in rats
- VernacularTitle:~(125)I粒子植入对大鼠脑内胶质瘤细胞增殖的抑制作用
- Author:
Haiyan HUANG
;
Xinyu HONG
;
Yang CHEN
;
Yinan LUO
;
Bai LI
- Publication Type:Journal Article
- Keywords:
SHG-44 cell;
stereotaxic techniques;
~ 125 Ⅰ;
proliferating cell nuclear antigen;
gene
- From:
Journal of Jilin University(Medicine Edition)
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possibility and mechanism of ~ 125 I in treatment of glioma. Methods SHG-44 glioma cells were cultivated in vitro, the inhibitory effect of ~ 125 I on SHG-44 cell proliferation was determined by MTT method. The stereotactic method was used to establish the rat intracranial glioma model. The MRI was performed at 1st week after implantation and ~ 125 I was implanted in the glioma area, the MRI was performed to measure the diameter of tumor 2 weeks after implantation. The rats were killed after 2 weeks ,PCNA gene experession was determined with immunohistological method both in control and experiment group.Results one week after implantation the glioma grew,the results of MTT method showed the growth of the SHG-44 was inhibited, ~ 125 I inhibited the expression of PCNA gene and enlonged the rat survival period. Conclusion ~ 125 I can inhibit the growth of glioma ,the mechanism may be concerned with its inhibitory effect on PCNA gene expression.
