Association between mannose-binding lectin gene polymorphism and frequent relapses in childhood nephrotic syndrome
- VernacularTitle:甘露糖结合凝集素基因多态性与儿童肾病综合征频复发的关联
- Author:
Yuanyuan GU
- Publication Type:Journal Article
- Keywords:
Mannose-binding lectin;
Gene polymorphism;
Childhood;
Nephrotic syndrome;
Frequent relapses
- From:
Journal of Medical Postgraduates
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine whether mannose-binding lectin(MBL) variant alleles were associated with frequent relapses in childhood nephrotic syndrome(NS). Methods: MBL alleles of codon 54 and promoter region at positions-550 and-221 were genotyped by means of Polymerase Chain Reaction-Restricted Fragment Length Polymorphism(PCR-RFLP)and PCR-Sequence-Specific Primers(PCR-SSP)assay in 32 China Han Nationality children with frequently relapsing NS(FRNS) and 31 with non-frequently relapsing NS(NFRNS),as well as in 32 healthy control subjects. Results: In patients with upper respiratory infections before relapses,the mutant genotype(GGC/GAC and GAC/GAC) frequency of MBL codon 54 was higher in children with FRNS(76.0%) than in children with NFRNS(42.9%).The B allele mutation frequency of MBL codon 54 was higher in children with FRNS than in healthy control subjects(34.4% vs 12.5%,P
