PER-1 Type Extended-spectrum ?-Lactamases Produced by Gram-negative Bacilli
- VernacularTitle:产PER-1型超广谱?-内酰胺酶革兰阴性杆菌的研究
- Author:
Fangyou YU
;
Meilan LI
;
Longhua HU
;
Kunru JIA
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
Klebsiella pneumoniae;
Enterobacter cloacae;
ESBLs;
PER-1;
Gene
- From:
Chinese Journal of Nosocomiology
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the prevalence of PER-1 type ESBLs-producing Enterobacteriaceae and their antimicrobial-resistance profile. METHODS Totally 167 strains of Escherichia coli, 154 strains of Klebsiella pneumoniae and 67 strains of Enterobacter cloacae were tested. All of them were isolated from all kinds of clinical specimens in four hospitals of Nanchang city from Aug 2003 to Jun 2004. Antimicrobial susceptibility test(AST) was determined by K-B disk diffusion test. double-disk test(CTX, CTX/CA and CAZ, CAZ/CA)and three- dimension extract test were used to determine ESBLs. PER-1 genes were amplified by PCR. The products of PCR were sequenced to identify its ?-lactamase encoding gene. RESULTS Sixteen isolates were PER-1 gene positive. The PER-1 gene positive rates of E. coli, K. pneumoniae and E. cloacae were 3.6%, 3.2% and 9.0% , respectively . The antimicrobial-resistant rates of isolates harboring blaPER-1 to cefotaxime, ceftaxidime, amikacin , cefoxitin , cefepime and imipenem were 100%,100%, 56%, 94%, 31% and 0%, respectively. CONCLUSIONS blaPER-1 is detected in E. coli, K. pneumoniae and E. cloacace, and has diffused in Enterobacteriaceae . Imipenem and amikacin may be used to treat the infection caused by bacteria harboring blaPER -1.
