Clinical application of anti-proteinase 3 capture ELISA in diagnosis of Wegener's granulomatosis
- VernacularTitle:捕获法ELISA检测抗蛋白酶3抗体对韦格纳肉芽肿的临床应用价值
- Author:
Zhenru FENG
;
Ping LIU
;
Baohuan SUI
- Publication Type:Journal Article
- Keywords:
capture ELISA;
classic ELISA;
anti-proteinase 3;
Wegener's granulomatosis
- From:
Chinese Journal of Clinical Laboratory Science
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investige the sensitivity and specificity of anti-proteinase 3 (PR3) capture ELISA in diagnosis of Wegener's granulomatosis (WG),and the correlation between the capture ELISA and indirect immunofluorescence assay (IIF).Methods Anti-PR3 antibody and anti-neutrophil cytoplasmic antibody (cANCA) in sera from 72 patients with WG,206 healthy blood donors and 24 patients with autoimmune diseases were detected by classic ELISA,capture ELISA and IIF.Results The sensitivities of classic ELISA and capture ELISA for detection of anti-PR3 in WG diagnosis were 73.6% and 87.5% respectively.The specificities of both the ELISAs were identical (100%).Detection of anti-PR3 by ELISA or IIF alone led to the serological hit rate of 87.5% and 84.7% for WG respectively,but the combination of capture ELISA and IIF increase the hit rate up to 91.6%.Conclusions The sensitivity of anti-PR3 capture ELISA as well as its correlation with IIF is prior to classic anti-PR3 ELISA.The combined detection of anti-PR3 capture ELISA and IIF may increase the diagnosis rate of clinically suspected WG.
