Culture,purification and differentiation in vitro of human retinal progenitors
- VernacularTitle:人视网膜前体细胞的体外培养、纯化和诱导分化
- Author:
Jing MA
- Publication Type:Journal Article
- Keywords:
retinal progenitors;
culture;
neural stem cells;
identification;
modulation
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expansion and purification of human retinal progenitors cultured in vitro.(Methods Human) embryonic retinal progenitor cells(RPCs) were cultured.The characteristics of neural stem cell were observed.The expression of Nestin was examined flow cytometry(FCM) for the comparsion between the primary and secondary passages.The differentiation of RPCs had been observed after that they had been cultured in different conditions.Results Human RPCs had proliferated and formed cell clusters with a shape of neurosphere.After digestion,the scattered progenitors formed secondary neurosphere-like cell clusters.The cells in those clusters expressed Nestin and were negative for the mature retinal cell proteins.After spontaneous differentiation,the retinal progenitors expressed special proteins of mature retinal cells,including neuron specific enolase(NSE),glial fibrillary acidic protein(GFAP),protein kinase C(PKC) and Syntaxin-1.The expression of Nestin in the secondary culture passage was(39.3?18.9)%,which was significantly higher than that of the primary passage [(28.7?17.7)%](P
