Trophic effects of mouse Sertoli cells on cerebral cortical cells in vitro
- VernacularTitle:小鼠睾丸支持细胞对大脑皮质细胞的体外营养作用
- Author:
Jie JIN
;
Xiaolin SHI
- Publication Type:Journal Article
- Keywords:
cell culture;
Sertoli cell;
cerebral cortex;
neuron;
neural stem cell
- From:
Basic & Clinical Medicine
2006;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible way through which transplanted Sertoli cells support the nerve tissues in vivo.Methods Culture the isolated cells from the fetal mouse cerebral cortex alone(coated the culture plate with poly-D-lysine previously),the control group,or with prepubertal homologous Sertoli cells(planted Sertoli cells beforehand),the coculture group.To observe the morphological changes of the cultured neurons,neural stem cells(NSC) and neuroglial cells every day and compared the differences in their growth between the tow groups.Sertoli cells,neurons and NSC were identified by the expression of Fas ligand,neuron-specific enolase(NSE) and Nestin in the cytoplasm respectively.Results A significant increase of both the survival of neurons(P
