Change of Cytoskeleton and Variance of Ca~(2+) in Cultured Cells During the Invasion of Toxoplasma gondii
- VernacularTitle:刚地弓形虫入侵细胞过程中细胞骨架和Ca~(2+)浓度变化
- Author:
Liwei LI
;
Zhexin SHAO
;
Ji YAN
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
HUVEC;
J774A.1;
Cytoskeleton;
Ca~(2+)
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the change of cytoskeleton and the variance of Ca2+ in cultured cells during the invasion of Toxoplasma gondii. Methods Tachyzoites suspensions were gathered by routine method and used to infect phagocytic cells(J774A.1)and non-phagocytic cells (HUVEC). The ability of T. gondii invading into the cells and the influence of cytoskeleton inhibitor,colchicine and cytochalasin D,were observed by microscopy. The rearrangement of cytoskeleton of cells was observed by fluoromicroscopy. By using laser scanning confocal microscope,the variance of Ca2+ in J774A.1 and HUVEC was detected. Results Ca2+ increased greatly in J774A.1 during the invasion of T.gondii(P0.05). The microfilaments of J774A.1 were agglomerated during the invasion of T. gondii. Cytoskeleton inhibitor,cytochalasin D(P0.05)during the invasion and cytoskeleton was not changed. Cytochalasin D and colchicine showed little effect on the infection rate of HUVEC. Conclusion The concentration of Ca2+ increases greatly and cytoskeleton(mainly the microfilament)has been rearranged in phagocytic cell during the invasion of T. gondii,while both of them show no significant change in non-phagocytic cell.
