Molecular Identification of Naturally Acquired Plasmodium knowlesi Infection in a Human Case
- VernacularTitle:自然感染诺氏疟原虫患者血片样本PCR鉴定
- Author:
Hui ZHENG
;
Huaimin ZHU
;
Beifang NING
;
Xiangy LI
- Publication Type:Journal Article
- Keywords:
Plasmodium knowlesi;
Natural infection;
Human;
SSU rRNA
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To confirm the diagnosis of a human case with atypical vivax-malaria from Yunnan Province by molecular technique. Methods DNA was extracted from blood films of unidentified sample, and of four known Plasmodium species (P. vivax, P. falciparum, P. knowlesi, and P. cynomolgi). A DNA-based diagnosis with the polymerase chain reaction (PCR) method targeting the small subunit ribosomal RNA (SSU rRNA) genes of genus- and species-specific (two human malaria species and P. knowlesi) was introduced. Results The PCR amplification with primer pair specific for P. knowlesi produced a single fragment of 150 bp. Sequence analysis showed that the amplified fragment was identical to the sequence of P. knowlesi. Conclusion The patient was naturally infected with P. knowlesi.
