Construction of Suppression Subtracted cDNA Library of Deltamethrin-resistant Aedes albopictus
- VernacularTitle:溴氰菊酯抗性白纹伊蚊抑制消减cDNA文库的构建
- Author:
Jiahong WU
;
Tongyan ZHAO
;
Yand DONG
- Publication Type:Journal Article
- Keywords:
Aedes albopictus;
Deltamethrin;
Resistance;
Suppression subtractive hybridization;
Gene library
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct the suppression subtracted cDNA library of deltamethrin-resistant Aedes albopictus. Methods Total RNA was extracted from the deltamethrin-resistant (R-lab) and -sensitive (S-lab) isolates, mRNA was obtained after purification. Double stranded cDNAs were synthesized after reverse transcription. Two subtractions were performed by suppression subtractive hybridization with S-lab as tester and R-lab as driver or S-lab as driver and R-lab as tester. Enriched different expressed cDNA was cloned into pMD18-T vector to construct subtractive libraries. Results The subtracted cDNA libraries contained 580 and 477 positive clones respectively. The PCR results of 150 clones picked randomly from each library showed that the positive ratio of constructed cDNA libraries was 93%, with a length of cDNA fragments ranged from 150 bp to 750 bp. Conclusion The suppression subtracted cDNA library of deltamethrin-resistant Ae. albopictus is constructed.
