DNA Amplification of Plasmodium vivax Parasites from Giemsa-stained Blood Smears
- VernacularTitle:从Giemsa染色的血膜中扩增间日疟原虫DNA方法探讨
- Author:
Fangzhen XIAO
;
Shanying ZHANG
;
Longshan XU
;
Jianghong HUANG
;
Hanguo XIE
;
Yangrong OU
- Publication Type:Journal Article
- Keywords:
Blood smear;
Plasmodium vivax;
DNA extraction
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop methods of extracting DNA from malaria parasites on Giemsa-stained blood smears. Methods Improved Na2HPO4 method and Chelex-100 ion-exchange technique were used to extract DNA from Giemsa-stained or unstained blood smears. Nested PCR was employed for amplification and identification of allelotypes in the Plasmodium vivax merozoite surface protein-1(PvMSP-1). Results Target DNA bands appeared in all samples of unstained thick blood smears, while no DNA bands were visible in the fixed and stained thin smears. Both methods identified PvMSP-1 alleles from smears with parasitemia of ≥0.01%. Conclusion It is feasible to identify PvMSP-1 alleles from Giemsa-stained blood smear.
