CTL Response to Pre-erythrocytic Stage Vaccine Candidate of Plasmodium falciparum in HLA-A*0201 Transgenic Mice Detected by ELISPOT Assay
- VernacularTitle:ELISPOT检测HLA-A*0201转基因小鼠对恶性疟原虫红前期候选抗原的CTL应答
- Author:
Feng QIAN
;
Qingfeng ZHANG
;
Luhui SHEN
;
Weiqing PAN
- Publication Type:Journal Article
- Keywords:
Plasmodium falciparum;
Vaccine;
Pre-erythrocytic stage;
CTL;
ELISPOT assay
- From:
Chinese Journal of Parasitology and Parasitic Diseases
1987;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
The importance of cytotoxic T-lymphocyte(CTL)against malaria parasite in pre-erythrocytic stage has been presented in relevant researches. In order to investigate whether one CTL epitope(YLNKIQNSL)involved in a chimeric pre-erythrocytic stage vaccine candidate of Plasmodium falciparum which was expressed and purified in the laboratory can stimulate in vivo CTL response,HLA-A*0201 transgenic mice were immunized with this vaccine candidate. Enzyme-linked immunosorbent spot(ELISPOT)assay was performed on the splenocytes from the immunized transgenic mice. Positive result indicated that this CTL epitope can be in vivo processed and correctly presented.
