Analysis of the UV absorbing constituents of the metabolites from UV-B tolerance bacteria and study on its anti-ultraviolet mechanism
- VernacularTitle:抗UV-B辐射菌紫外吸收代谢产物分析及其抗紫外辐射活性研究
- Author:
Hongyuan WANG
;
Xiaolu JIANG
;
Hong REN
;
Xiaoting LIANG
;
Huashi GUAN
- Publication Type:Journal Article
- Keywords:
Pantoea agglomerans;
anti-ultraviolet radiation;
2,3-butanediol;
UV-B;
metabolites
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The anti-UV-B radiation mechanism of UV-B tolerance strain KFS-9 was studied from the profile of metabolites.Methods The compounds were separated by column chromatography and their structures were elucidated based on GC-MS,LC-TOF-MS,EI-MS and NMR analyses.Results Three unsaturated fatty acids(identified as 9-hexadecenoic acid,9,12-octadecadienoic acid and 11-octadecenoic acid) and 1,2-benzenedicarboxylic acid able to absorb ultraviolet were isolated from the petroleum ether extract of the fermentation liquid of Pantoea agglomerans KFS-9.Fraction(Ⅱ) was isolated from the ethyl acetate extract and was composed of 2,3-butanediol and a series of high unsaturated aroma compounds.Fraction(Ⅱ) had a wide absorption peak,and it could protect E.coli from UV-B damage in some sense.Conclusion Strain KFS-9 produced metabolites that were able to absorb UV to build a natural barrier and so improved the tolerance to UV radiation.The UV-B radiation protection test to the E.coli also showed fraction(Ⅱ) was not the only protector,and there definitely existedother materials and mechanism to protect the strain.
