Protection of phycocyanin on the neurons and its effects on the expression of Caspase-3 genes after cerebral ischemia-reperfusion in rats
- VernacularTitle:藻蓝蛋白对大鼠脑缺血再灌注损伤的保护作用及对Caspase-3 mRNA表达的影响
- Author:
Hongbing CHEN
- Publication Type:Journal Article
- Keywords:
phycocyanin;
cerebral ischemia;
reperfusion injury;
gene expression,Caspase-3
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protection of phycocyanin on the neurons and the expression of Caspase-3 genes after cerebral ischemia-reperfusion in rats.Methods The model of middle cerebral artery occlusion/reperfusion(MCAO/R) was established using intraluminal filament method.All 84 rats were randomly divided into sham operation group(4 cases),control group(40 cases) and therapeutic group(40 cases) treated by phycocyanin.The nervous functional deficit score was evaluated by Bederson's testing,the cererbral infarction volume was measured using 2,3,5-triphenyltetrazolium chloride(TTC) staining,and the expression of Caspase-3 mRNA was determined by in situ hybridization at regular intervals after ischemic reperfusion.Results Phycocyanin could improve nervous functional deficit score and reduce the cerebral infarction volume during 24～48h after cerebral ischemia-reperfusion in rats.In the control group,the upregulation of Caspase-3 mRNA began 6h after reperfusion,reached its maximum at 24h and subsided gradually from 48h to 14d.In the therapeutic group,the time-phase pattern of Caspase-3 mRNA expression was similar to that in the control group,and the number of caspas-3-positive cells were significantly lower than that in the control group at the same time.Conclusions Phycocyanin could inhibit the over-expression of Caspase-3 mRNA in neurons,and might play an important role in protecting ischemic reperfusion injury in rats.
