Isolation and structure identification of bioactive fraction AM105-Ⅱ from marine actinomycete
- VernacularTitle:海洋放线菌活性组分AM105-Ⅱ的分离与结构鉴定
- Author:
Huiqin HUANG
- Publication Type:Journal Article
- Keywords:
micromonospora;
antibiotic;
isolation and purification;
structure identification;
rifamycin
- From:
Chinese Journal of Marine Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate and identify the anti-MRSA bioactive fraction from the fermentation broth of the marine-derived actinomycete AM105.Methods By means of silica gel column chromatography,preparative TLC and Sephadex LH20 column chromatography,the bioactive component was purified.The structure was determined by the spectral methods.Results and Conclusion The bioactive component AM105-Ⅱ was purified and the spectra showed that AM105-Ⅱ was composed of rifamycin S and its isomer,named isorifamycin S,which is a novel structure compound.Its antibacterial activity is better than rifamycin SV according to MIC.
