Emodin inhibit the migration and proliferation of vascular smooth muscle cell independent on metabolism
- VernacularTitle:大黄素自身抑制血管平滑肌细胞迁移和增殖
- Author:
Xiangfei WANG
;
Junbo GE
;
Aijun SUN
- Publication Type:Journal Article
- Keywords:
Emodin;
Muscle smooth vascular;
Cell
- From:
Chinese Journal of Interventional Cardiology
1996;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of emodin on the migration and proliferation of vascular smooth muscle cell (VSMC) and the metabolism of emodin in VSMC. Methods The effects of emodin on the migration or proliferation of vascular smooth muscle cell were measured by “transwell" migration system and MTT assay. Results The migration of VSMC could be significantly inhibited by emodin and the inhibitory ratio was 83.8% in 5 ?g/mL emodin group. The antiproliferative effect of emodin was in a dose- and time- dependent manner. However, the supernatant concentration of emodin insignificantly dereased after culture for 24 h. The cytotoxicity of emodin and cellular ROS was not influenced by CYP inducer or inhibitor. The mRNA expression of emodin's primary metabolic enzyme (cytochrome p450 oxidase, CYP) up-regulated insignificantly after treatment of emodin for 24 h. Conclusion Emodin can inhibit the migration and proliferation of VSMC and can not be metabolized by VSMC. Emodin may be a choice for the medication of drug-eluting stent.
