External quality assessment on antinudear antibody detection
- VernacularTitle:抗核抗体谱检测的室间质量评价
- Author:
Lunan WANG
;
Wei DENG
;
Jinming LI
- Publication Type:Journal Article
- Keywords:
Antibodies, antinuclear;
Quality control;
Autoimmune diseases
- From:
Chinese Journal of Laboratory Medicine
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the performance of antinuclear antibody (ANA) detection in clinical laboratories. Methods There were 2 external quality assessments ( EQA) for nuclear antibody detection. The panel consisting of 5 liquid serum samples was distributed. Each participants of the EQA program should include in their report the results, the methodological procedure and the kits used . All data were analyzed then distributed to all laboratories in our EQA program. Results During 2003 to 2005, more than 70% laboratories detected antinuclear antibody by using indirect immunofluorescence(IIF) method and the rate of acceptable response list were 94. 1% , 93. 0% and 98. 6% , respectively. We have got better result from signals fluorescence pattern such as homogeneous and speckled than multiplicity samples. A wide dispersion of ANA titers was obtained every year. We neceived different rate from different methods of anti-ENA detection and 90% laboratories used immunoblot method. For anti-dsDNA most participants gave us acceptable performance. Conclusions ANA detection in routine practice is far from being standardized. However, EQA can have an impact on ANA detection performance if it provides advice for participating laboratories to limited laboratory variations related to method, procedure and kits.
