Study on quinolone-resistance mechanism and the REP-PCR fingerprinting in Salmonella Typhimurium
- VernacularTitle:鼠伤寒沙门菌对喹诺酮类耐药机制研究与基因分型
- Author:
Jing CHEN
;
Ziyong SUN
;
Zhenlin ZHANG
- Publication Type:Journal Article
- Keywords:
Salmonella typhimurium;
Quinolones;
Ploymerase chain reaction
- From:
Chinese Journal of Laboratory Medicine
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the resistance of Salmonella enterica serotype Typhimurium (STM) to quinolone. Methods A total of 33 salmonella typhimurium resistant to Ciprofloxacin in stool of outpatients from July 1st. 2004 to Oct 31st was isolated. in four hospitals in Wuhan. Antimicrobial susceptibility test was conducted by the Agar Dilution method. All of the strains were studied for the occurrence of mutations in the genes coding for QRDR by PCR and REP-PCR. Results The sequencing of QRDR of these genes in highly quinolone-resistant mutants (MICs of 4 to 16?g/ml) of the 25 STM revealed the presence of gyrA mutation, and inserted base in the gyrB and parE genes were found only in 5 STM. With aid of REP-PCR, the 32 strains were divided into 9 profiles which were correlated to the antibiotic susceptibility spectrums. Conclusions The resistance of STM from community acquired infection in WuHan is very severe. The resistance mechanism was associated with mutations of the quinolone resistance-determining regions. It suggests an outbreak in the community.
