Study on mutation of pbp5 of ampidllin resistance in enterococcus faedum
- VernacularTitle:屎肠球菌对氨苄西林耐药pbp5基因突变的研究
- Author:
Leyan TONG
;
Liyan MA
;
Shuzhen XU
- Publication Type:Journal Article
- Keywords:
Enterococcus faecium;
Ampicillin resistance;
beta Lactamase;
Gene
- From:
Chinese Journal of Laboratory Medicine
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship of ampicillin resistance and?lactamase production, and gene mutation on pbp5 gene fragment in Enterococcus faecium. Methods?lactamase production was tested with nitrocefin, the pbp5 gene fragment of 57 isolates of ampicillin-resistant Enterococcus faecium (ARE. faecium) was amplified by PCR, the mutation of amplified pbp5 gene was detected by single-strand conformation polymorphism(SSCP), the specific mutation types were confirmed by sequencing. Results?lactamase was negative in 57 isolates of ARE. faeciums; there were 3-6 gene mutation sites on pbp5 gene fragment, and the frequent mutation sites were the position 485 ,499 and 466'. Conclusion The gene mutation of pbp5 gene fragment may play an important role in the ARE. faecium.
