Effect of Cyclosporine A on the immunoligical reject reaction of brain cell transplantation in rats with experimental brain hemorrhage
- VernacularTitle:环孢菌素A对脑细胞移植治疗大鼠实验性脑出血免疫反应的影响
- Author:
Xiaoming LI
;
Jun CAI
;
Ning LIU
- Publication Type:Journal Article
- Keywords:
Cyclosporin A;
brain cell transplantation;
reject reaction;
T lymphocyte
- From:
Journal of Clinical Neurology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of Cyclosporine A on the brain immunoligical reject reaction of transgenic cellular transplant in rats with experimental brain hemorrhage.Methods We established the brain hemorrhagic models of rats and the models were divided into group A (treated with Cyclosporin A) and group B (without treatment) randomly after transplanting brain cells modified by NGF gene. The levels of CD~+_4, CD~+_8T cells subsets in peripheral blood were examined by using flow cytometry at 15 d after transplantation. The expressions of MHC-classⅡantigens and infiltration of CD~+_4, CD~+_8T lymphocyte subsets were examined by SP immunocytochemical stain.Results The count of CD~+_4, CD~+_8T lymphocyte and CD~+_4/CD~+_8 in peripheral blood were 29.20?3.97, 20.65?2.02 and 1.41?0.86 in group A, and were 47.39?3.01, 28.30? 2.36 and 1.68?0.69 in group B, respectively.There were significant differences between the two groups (all P
