Study on the differentiation of neural stem cells in hippocampus of rat model of Parkinson's disease
- VernacularTitle:帕金森病大鼠海马区增殖神经干细胞分化情况的研究
- Author:
Lei WANG
;
Shuilin ZENG
;
Jianbao ZHU
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
rats;
neural stem cells;
differentiation;
hippocampus
- From:
Journal of Clinical Neurology
1995;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the differentiation of neural stem cells (NSCs) in hippocampus of rat model of Parkinson's disease (PD).Methods Rat models of PD were made by injection 6-hydroxydopamine (6-OHDA) into striatum. 5-bromodeoxyuridine (Brdu) was injected into the abdomens of PD rats for 14 days before they were killed. The expressions of Brdu/Glial fibrillary acidic protein (GFAP) and Brdu/ Neuronal nuclear antigen (Neun) were determined by immunocytochemistry. The distinctive marker for Brdu/GFAP and Brdu/Neun labeling method was used to determine the phenotype of the differentiated cells.Results After PD models were established, the Brdu/GFAP and Brdu/Neun positive cells appeared at 7 d, increased gradually at 14 d, and peaked at 28 d. In these double labelled cells, Brdu/GFAP positive cells were more than Brdu/Neun positive cells.Conclusions Direct injection of 6-OHDA into the striatum stimulates the proliferation of inherent NSCs in hippocampus of rats. Large amounts of NSCs differentiate into astrocytes and small amounts of them turn into neurons.
