The construction of human Eotaxin and its prokaryotic expression vector
- VernacularTitle:Eotaxin原核表达载体的构建及初步表达
- Author:
Jinchuan ZHOU
- Publication Type:Journal Article
- Keywords:
Eotaxin;
RT-PCR;
Sequence analyzing
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct prokaryotic cell expression vector of human Eotaxin for expressing and examining its activity,and for constructing the N-terminal mutations of human Eotaxin.Methods: Extract the total RNA from peripheral monocyte,and Eotaxin gene coding region was obtained by RT-PCR.The fragment was sequenced and analysed after cloning into T vector,then clone to prokaryotic cell expression vector-PET30a~+ and expressed in BL21(DE?3).Results:The right cloning of human Eotaxin and its prokaryotic cell expression were obtained.Conclusion: The construction and prokaryotic cell expression of human Eotaxin lay a foundation for its more expressing and examining their activity,and for constructing the N-terminal mutations of human Eotaxin.
