Effects of Fenofibrate on neonatal rat hypertrophic cardiomyocyte in vitro
- VernacularTitle:非诺贝特对新生大鼠体外肥大心肌细胞的影响
- Author:
Yongyao YANG
- Publication Type:Journal Article
- Keywords:
Peroxisome proliferator-activated receptor ?;
Fenofibrate;
Cardiac hypertrophy
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the inhibitory effects of peroxisome proliferator-activated receptor ? activator Fenofibrate on the angiotensin Ⅱ(Ang Ⅱ)-induced cardiac hypertrophy in vitro. Methods: A model of hypertrophy of neonatal rat cardiac myocytes was established with Ang Ⅱ stimulation.With the aid of Leca Qwin Image software,the surface areas of cardiac myocytes were analyzed.The mRNA expression of ?、?-myosin heavy chains(?-MHC、?-MHC) and PPAR? was measured by reverse transcription-polymerase chain reaction(RT-PCR),and the cultured myocyte viability was estimated by MTT assay. Results:Fenofibrate pretreatment 24 h prior to Ang ⅡSignificantly reduced Ang Ⅱ-induced cardiac hypertrophy,inhibited the effect of AngⅡ on the cardiac myocyte viability and increased expression of?/?-MHC mRNA and PPAR? mRNA in a dose-dependent manner.In contrast,Fenofibrate had no significant effect on Ang Ⅱ treated cardiac myocytes when Fenofibrate treatment was concomitant with Ang Ⅱ. Conclusion: PPAR?-dependent pathway was involved in the inhibition of cardiac hypertrophy,but chronic treatment was needed.
