Protective mechanism of Donepezil on apoptosis of PC12 cells
- VernacularTitle:多奈哌齐对PC12细胞凋亡抑制作用机制的研究
- Author:
Ke LI
- Publication Type:Journal Article
- Keywords:
Donepezil;
Amyloid beta protein;
Bcl-2;
Caspase-3
- From:
Journal of Medical Postgraduates
2003;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the protective mechanism of Donepezil on apoptosis of PC12 cells induced by amyloid beta protein. Methods: PC12 cells were cultured as previously,then amyloid beta protein and Donepezil were used to treat PC12 cells.Morphology observation,TUNEL and immunocytochemistry were employed to investigate the damage of PC12 cells induced by A?,protection of Donepezil on apoptosis of PC12 cells and the expression of Bcl2 and Caspase-3 in A?-treated group and Donepezil-treated group. Results: A? induced apoptosis of PC12 cells in a dose-dependent way;Compared to the group treated with A? only and the group post-treated with donepezil, the apoptosis index of PC12 cells(17.29?0.83)% and expression of Caspase-3(26.46?2.87)% were significantly downregulated in the group pretreated with donepezil(P0.05). Conclusion: This study demonstrated that Donepezil can protect PC12 cells against apoptosis induced with amyloid protein,which was closely related to the time of interference.The mechanism of protection was involved with the expression of Bcl-2 and Caspase-3.
