Inhibitory effect of heparanase antisense oligodeoxynucleotides on the invasiveness of human liver cancer cell lines
- VernacularTitle:乙酰肝素酶反义硫代寡脱氧核苷酸对人肝癌细胞系侵袭力的抑制作用
- Author:
Shunxiang WANG
;
Xiaohui WU
;
Shaoying ZHOU
;
Li PENG
- Publication Type:Journal Article
- Keywords:
heparanase;
antisense oligodeoxynucleotide;
hepatocarcinoma;
invasiveness
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of heparanase antisense oligodeoxynucleotide (AS-ODN) on the invasiveness of human liver cancer cell lines SMMC-7721,BEL-7402 in vitro. Methods We designed and synthesized AS-ODN that was complementary to the start codon region of heparanase mRNA,and the control,nonsense oligodeoxynucleotide (NS-ODN). The ODNs with the final concentration of 300 nmol/L was delivered into SMMC-7721,BEL-7402 cells by Oligofectamine TM Reagent. We evaluated heparanase gene expression using RT-PCR and detected heparanase protein expression using Western blot assay after transfection. Cell invasiveness was measured by Matrigel invasion assay.Results The result showed that heparanase gene expression,protein expression and invasiveness in AS-ODN group decreased significantly as compared with control groups( P
