Up-expression of vascular endothelial growth factor in human peritoneal mesothelial cells induced by ovarian carcinoma cells
- VernacularTitle:卵巢癌细胞诱导腹膜间皮细胞血管内皮生长因子表达增强
- Author:
Jingjing ZHANG
;
Bo WANG
- Publication Type:Journal Article
- Keywords:
ovarian neoplasms;
peritoneum;
metastases;
transforming growth factor?1;
vascular endothelial growth factor
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the impact of ovarian carcinoma cells on the expression of vascular endothelial growth factor(VEGF) in human peritoneal mesothelial cells(HPMC). Methods HPMC was incubated with ovarian carcinoma cell line in SKOV3 conditioned medium(SKOV3-CM) for(3～ 72 hrs ). RT-PCR was used to detect VEGF gene expression. VEGF protein expression in cell lysates was assessed by ELISA. HPMC was cultured for 24 hrs in SKOV3-CM or SKOV3-CM with anti-transforming growth factor?1(TGF-?1)neutralizing antibody,and the changes in VEGF gene and protein expression were examined. Results VEGF mRNA and protein were detected in HPMC without stimulation of SKOV3-CM. SKOV3-CM significantly induced HPMC VEGF mRNA and protein expression in a time-dependent manner and the induction started 6h(for mRNA) and 12 h (for protein) after the treatment(P
