Primary culture of human preadipocyte in a serum-free medium
- VernacularTitle:无血清原代培养人前脂肪细胞并诱导分化
- Author:
Huijuan ZHU
;
Jieying DENG
;
Fengying GONG
;
Yifan SHI
- Publication Type:Journal Article
- Keywords:
culture with serum-free medium;
primary culture;
preadipocyte;
proliferation;
differentiation
- From:
Basic & Clinical Medicine
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of primary culture of human preadipocyte in a serum-free medium. Methods Collangenase digestion was used to dispart preadipocyte.The cells were identified by microscopy stained by oil red O and determination of the activity of Glycerol-3-phosphate dehydronase(G-3-PDH). Results The primary cultured human preadipocyte proliferated in the aserum-free medium successfully.In the differential serum-free medium the cells turned to be round on the 4 th day.The adipose drops began to cumulate in the cells,and to the most quantity until the 21 st day.Conclusion The human preadipocyte can be primarily cultured and induced to differentiate in serum-free medium,which is the base for researching the effects of hormones and factors to the proliferation and differentiation of preadipocyte.
