Effect of benazepril on connective tissue growth factor mRNA expression in kidney of diabetic rats
- VernacularTitle:苯那普利对糖尿病大鼠肾脏结缔组织生长因子mRNA表达的影响
- Author:
Xianjun ZHAO
;
Cai LI
;
Xiangjun LI
;
Bo SUN
- Publication Type:Journal Article
- Keywords:
benazepril;
diabetes mellitus,experimental;
kidney;
connective tissue growth factor
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of benazepril on expression of connective tissue growth factor(CTGF)gene in kidney of diabetic rats and renal lesion.Methods Forty Wistar rats were randomly divided into control group(CON),diabetic group(DM) and diabetic rats treated with benazepril(DM+B) group.After benazepril was orally administered(15 mg?kg~(-1)?d~(-1))to the diabetic rats for 12 weeks,serum creatinine(Scr) and blood urea nitrogen(BUN) levels,urinary protein excretion(UAER)and angiotensin Ⅱ(AngⅡ) contents in renal cortex were determined.CTGF mRNA expression in the renal cortex was detected by RT-PCR.Immunohistochemstry was used to examine the expressions of fibronectin(FN) and type Ⅳ collagen(Col Ⅳ) in glomeruli.Results At the end of the experiment,UAER in DM group(12.92?3.56 mg?24 h~(-1)) was significantly higher than that in CON group(2.74?1.09 mg?24 h~(-1))(P
