Inhibitory effect of IGF-1 gene on motoneurons apoptosis in anterior horn after acute spinal cord injury in adult rats
- VernacularTitle:IGF-1抑制SCI大鼠脊髓前角神经元凋亡的体内实验
- Author:
Changfeng FU
;
Yi LIU
- Publication Type:Journal Article
- Keywords:
insulin-like growth factor Ⅰ;
spinal cord injuries;
apoptosis;
transgenes
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the effect of insulin-like growth factor-1(IGF-1) gene transfer on motoneurons apoptosis in anterior horn after acute spinal cord injury in adult rats.Methods Thirty-six male Wistar rats were randomly divided into 3 groups: IGF-1 group,model group and control group.The SCI models were made by cutting half spinal cord of rats on the T_8 segment by ophthalmogy scissors.Lipofectamine and recombinant plasmid pcDNA-hIGF-1 complexes were injected into the injury spinal cord by microinjector in IGF-1 group while pcDNA3.1,LipfectAmine and saline were administered in model group.The expression of IGF1 was detected by immunohistochemistry method,the apoptosis of the motoneurons in anterior horn was detected by TUNEL,and moting function of hindlimbs was evaluated by BBB locomotor scale.Results Compared with model group and control group,the expression of IGF-1 was increased significantly(P
