Correlations between peripheral benzodiazepine receptors in mitochondria,synaptosomes in brain cells and platelet membrane and biological significance
- VernacularTitle:血小板膜与脑细胞线粒体和突触体外周型苯二氮卓受体的相关性及生物学意义
- Author:
Chunfu CHEN
;
Senyang LANG
- Publication Type:Journal Article
- Keywords:
brain;
aging;
platelets;
mitochondria;
peripheral benzodiazepine receptors
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate correlations between peripheral benzodiazepine receptors(PBRs) in mitochondria and synaptosomes in the brain and platelet membrane of rats during aging and to explore the significance of the relationships.Methods Sprague-Dawley rats of both sexes were divided into 3-,12-and(24-month) groups.All animals were sacrificed by decapitation and the brains were immediately removed.Mitochondrial components from dissected cerebral cortex and synaptosomal fractions from dissected hippocampus were isolated by gradient centrifugation.The membrane of platelets from venous blood was prepared by the method of hypotonic haemolysis.The specific binding assay of the radioactive PBRs antagonist PK11195 to membrane was performed.(Results A significant) difference about PK11195 binding activity of mitochondria was observed among three groups(F=194.6,P
