Mobilizing effect induced by low dose irradiation on mouse peripheral blood stem/progenitor cells
- VernacularTitle:低剂量辐射对小鼠外周血干/祖细胞的动员作用
- Author:
Chunshui LIU
;
Guanjun WANG
- Publication Type:Journal Article
- Keywords:
radiation;
stem cells;
hematopoietic stem cell mobilization
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the mobilizing effect induced by low dose irradiation on mouse peripheral blood(stem/progenitor) cells.Methods Peripheral blood CFU-GM was cultivated in methylcellulose semi-solid culture system,and c-kit~+cells were counted by flow cytometry after low dose irradiation or combined with G-CSF.(Results The) CFU-GM and c-kit~+ cells of peripheral blood in irradiated mice were much higher than those in control mice,the CFU-GM started to increase at 24 h after irradiation,reached maximum at 72 h and stayed high level till 96 h.The CFU-GM and c-kit~+cells at 72 h after 75 mGy irradiation were the most.The CFU-GM and c-kit~+cells in semi-dose G-CSF+75 mGy group were much higher than those in simple low dose irradiation group and were close to those in the group dealed with adequate G-CSF.Conclusion Low dose irradiation can mobilize peripheral blood stem/progenitor cells,and low dose irradiation combined with G-CSF can produce synergetic effect.
