Targeting therapy of SLE associated thrombocytopenia with labeled antibody in vitro
- VernacularTitle:药物标记抗体体外导向治疗系统性红斑狼疮所致血小板减少
- Author:
Zhenyu JIANG
;
Lili YU
;
Mengyuan CAO
;
Cheng YAO
- Publication Type:Journal Article
- Keywords:
labeled antibody;
receptor,Fc;
immunoglobulins,intravenous;
lupus erythematosus,systemic;
thrombocytopenia
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the feasibility and effect of labeled antibody on targeting therapy of systemic lupus erythematosus(SLE) associated thrombocytopenia.Methods MTX-IVIG conjugate was prepared by indirect and direct cross linking,and the binding ability of it to Fc fragment was detected by indirect immunofluor-escence.The killing activity of the conjugate was detected by MTT method using murine macrophage with Fc receptor and strain U937 of human monocytic leukemia cell as targets.Results Conjugation showed stronger cytotoxicity upon target cells than free MTX,and it showed less cytotoxic effect on Fc receptor negative cells compared with the positive ones.IVIG-HSA-MTX restrained the phagocyte of macrophage.The killing effect of IVIG-HSA-MTX was significantly stronger than that of IVIG-MTX.Conclusion The conjugation can show a highly specific cytotoxicity upon mononuclear-macrophage in vitro.
