Biocompatibilities of bone marrow stromal cells and chitosan/tricalcium phosphate
- VernacularTitle:骨髓基质细胞与壳聚糖-聚磷酸三钙复合材料的生物相容性
- Author:
Li ZHANG
;
Ning MA
- Publication Type:Journal Article
- Keywords:
chitosan;
tricalcium phosphate;
bone marrow stromal cells;
tissue engineering;
biocompatible materials
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of chitosan/tricalcium phosphate(CTCP)on the attachment and proliferation of bone marrow stromal cells(BMSCs)cultivated in vitro and explore their biocompatibilities.Methods The CTCP was prepared by freeze-drying.BMSCs were cultivated in vitro,collected and seeded onto the surface of CTCP.Cell attachment and proliferation were obesrved using inverted light microscope and scanning electron microscopy(SEM).The cell proliferation was tracked by MTT method 2,4,6,8 d after seeding.Results BMSCs were fibroblast-like 2 d after seeding.The mean number of cells was(360?20) and(76?18),respectively,in the experimental and control group per 100 eyesight field,the difference was significant(P
