Influences of LHRH-PE40 on proliferation inhibition and apoptosis of human colon carcinoma cells
- VernacularTitle:LHRH-PE40对人结肠癌细胞增殖抑制和凋亡的影响
- Author:
Yang JIANG
;
Yan SONG
- Publication Type:Journal Article
- Keywords:
LHRH-PE40;
colonic neoplasms;
apoptosis;
receptors,LHRH
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To research the binding specificity of new recombination toxin LHRH-PE40 and LHRH receptor on the surface of human colon carcinoma cell line Lovo and the mechanism of anti-proliferation,and determine the apoptosis.Methods Lovo cells were analysed by LHRH-PE40 marked with()~(125)Ⅰ;the cytocidal effect of the anti-tumor was evaluated by MTT assay,and the apoptotic rate was analysed by flow cytometry.Results Lovo cells had the binding of aglucone and receptor.Half lethal dose of human colon carcinoma cells Lovo with LHRH-PE40 was 0.24 mg?L~(-1).The apoptotic rate was increased when the LHRH-PE40 concentration was ranged from 0.1 to 10 mg?L~(-1)(P
