Correlation between chromosome fragile site and proto-oncogene in patients with hepatocellular carcinoma
- VernacularTitle:肝癌患者淋巴细胞染色体脆性部位与原癌基因的相关性
- Author:
Yan CAO
;
Hong LIU
;
Yonghong LIU
- Publication Type:Journal Article
- Keywords:
liver neoplasms;
chromosome fragility;
proto-oneogenes;
lymphocytes
- From:
Journal of Jilin University(Medicine Edition)
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the correlation between chromosome fragile site in peripheral blood lymphocytes and proto-oncogene expression rate in patients with hepatocellular carcinoma.Methods The peripheral blood lymphocyte chromosome preparation was made routinely.The cells were cultivated with low concentrations of calf serum and folic acid,higher pH medium and G-banding method.The fragile sites in 20 patients with hepatocellular carcinoma(experimental group) and 18 healthy subjects(control group) were exactly located and analyzed.(Results The) fragile sites in experimental group were determined as 44 species mainly distributed on the chromosomes of A,B,C group.The expression rate of the fragite site in experimental group(9.90%) was significantly higher than that in control group(0.67%)(P
