Analysis of HIV-1 Clade B Nef specific CD8~+ T cell inter-clade cross-reactivity to Clade B and C antigen in Chinese population
- VernacularTitle:人类免疫缺陷病毒-1B和C亚型Nef蛋白特异性CD8~+ T细胞交叉应答反应的研究
- Author:
Yan ZHUANG
- Publication Type:Journal Article
- Keywords:
Human immunodeficiency virus-1;
Clade B,Clade C;
Genes,nef;
CD8~(+)T cell response;
Cross-reactivity
- From:
Journal of Medical Postgraduates
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To analyse the character of HIV1 Clade B Nef specific CD8~(+)T cell inter-clade cross-reactivity to clade B and C antigen in Chinese population. Methods: PBMC from 51 HIV-1 Clade B infected donors were separated by Ficoll-Hypaque density gradient centrifugation.HIV-1 Nef specific CD8~(+)T cell responses were analyzed by gamma interferon(IFN-?) enzyme-linked immunospot(ELISpot) assay using 54 synthetic peptides spanning all HIV-1B and C Nef proteins. Results:(82.9%)(29/35)of the individuals who had the Nef specific CD8~(+)T cell responses could recognize both Clade B and Clade C peptides.There was no significant difference between the two Clades on number of the recognized peptides and magnitude of the responses(P=0.529,P=0.754).70.4% Nef peptides either of Clade B or of Clade C could be recognized by HIV-1 B Nef specific CD8~(+)T cell.The sequence homology of peptides which could be cross-recognized was significantly higher than that of peptides which could be single-recognized. Conclusion: There are broadly cross-reactive T cells in HIV-1 Clade B infected individuals which can recognize both Clade B and Clade C Nef antigen.These cross-reactive T cells recognize peptides with higher inter-clade homology.
