A study on mechanism for resistance of carbapenems in Acinetobacter baumannii
- VernacularTitle:鲍曼不动杆菌对碳青霉烯类抗生素耐药机制研究
- Author:
Chunyu LU
;
Zheng ZHANG
- Publication Type:Journal Article
- Keywords:
Acinetobacter baumannii;
resistance;
?-lactamase;
Carbapenems
- From:
Chinese Journal of Clinical Laboratory Science
1985;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of Acinetobacter baumannii resistant to carbapenems.Methods E test and microdilution were used to determine the minimum inhibitory concentrations(MIC) of 11 antibiotics against 30 isolates of imipenem-resistant Acinetobacter baumannii strains.The production of ?-lactamase was detected by improved three-dimensional test.Nine coding genes of?-lactamase were detected by PCR.Results The resistance rates of 11 antibiotics were over 90% except for polymyxin B(23.3%) and ciprofloxacin(87.7%).Among 30 isolates of Acinetobacter baumannii the positive rate of single ESBLs,single AmpC,ESBLs+AmpC,non-ESBLs non AmpC?-lactamase was 33.3%(10/30),3.3%(1/30),23.3%(7/30) and 26.7%(8/30) respectively by three-dimensional test.The coding genes:VIM-1,VIM-2,OXA-24,CTX-M-2,IMP-1 and VEB-1 were undetectable.Two or three of coding genes:OXA-23,PER-1 and AmpC were detectable in 27 isolates,and their homologies compared with corresponding sequences of GenBank were more than 98%.A nucleotide mutation(A→C) at 617 site of PER-1 gene was found in No.26 strain,so this sequence was registered in GenBank(Accession No.DQ341275).Conclusions Carbapenems resistance in Acinetobacter baumannii related with the production of OXA-23,PER-1 and AmpC.
