VIM-2 Metallo-?-lactamase Producing Pseudomonas aeruginosa in Burn Wards
- VernacularTitle:产VIM-2型金属酶铜绿假单胞菌的研究
- Author:
Rui CHEN
;
Xiangmin PENG
;
Jiaxin ZHU
;
Yingchun TANG
;
Hongyu LI
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Metallo-?-lactamase;
Sequence homology;
Nucleic acid
- From:
Chinese Journal of Nosocomiology
2006;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To analyze homology,antimicrobial susceptibility and metallo-?-lactamase gene type of metallo-?lactamase producing Pseudomonas aeruginosa in burn wards.METHODS Antimicrobial susceptibility tests were performed with E-tests.Using 2-mercaptoethanol disc synergy test to screen metallo-?-lactamase(positive) strains from imipenem-resistant P.aeruginosa in burn wards.Metallo-?-lactamase gene and integrase gene were amplified and sequenced.Resistance plasmid transfer and curing experiments were implemented to study the transfer of imipenem resistance and plasmid DNA was extracted and purified with Qiagen Plasmid Mini Kit.Random amplified polymorphic DNA(RAPD) typing was carried out to analyze the homology of the isolates.(RESULTS) Six strains of P.aeruginosa were suggested to produce metallo-?-(lactamase) by 2(-mercaptoethanol) disc synergy test.Using primers described for bla(VIM),the amplifications were observed among all 6 isolates and a VIM-2 metallo-?-(lactamase) gene was identified by sequencing.All isolates which producing VIM-2 metallo-?-(lactamase) had class 1 integrase gene and derived from a same clonal origin.CONCLUSIONS(VIM-2) metallo-?-(lactamase) producing P.aeruginosa is prevalent in burn wards,all isolates which producing VIM-2 metallo-?-(lactamase) have class 1 integrase gene.
